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Research Goal:
tidentification and characterization of two forms of the
Disabled-1 (Dab1) gene: Dab1-early expressed in retinal
progenitor cells and Dab1-late expressed in two classes of
neuronal cells (amacrine and ganglion) in the developing retina.
Techniques: RNA extraction, RT-PCR, western
blot analysis, protein-RNA immunoprecipitation, transfection of
primary retinal cultures, site-directed mutagenesis, confocal
microscopy, immunostaining of retinal tissue sections, in situ
hybridization of retinal tissue sections.
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Publications:
Godbout, R., Packer, M., Katyal, S. and Bléoo, S. Cloning and
expression of the chicken DEAD box gene DDX1. Biochim. Biophys. Acta
1574:63-71 (2002).
Godbout, R., Andison, R., Katyal, S. and Bisgrove, D.A. Isolation of
a novel cDNA enriched in the undifferentiated chick retina and lens.
Dev. Dyn. 227: 409-415 (2003).
Katyal, S. and Godbout, R. Dab1 alternative splicing modulates the
disabled (Dab1) pathway in the developing chick retina. EMBO J. 23:
1878-1888 (2004).
Godbout, R. and Katyal, S. DEAD box genes in chicken. Second Report
on Chicken Genes and Chromosomes 2005. Cytogenet. Genome Res. 109:
459-462 (2005).
Godbout, R. and Katyal, S. Tissue distribution of DEAD box
transcripts in chicken. Second Report on Chicken Genes and
Chromosomes 2005. Cytogenet. Genome Res. 109: 462-466 (2005).
Presentations:
Katyal, S. and Godbout, R. Characterization of disabled-1 expression
in the developing chick retina. Alberta Cancer Board Annual Research
Meeting, Banff, Alberta (2000).
Katyal, S., Packer, M.K. and Godbout, R. Characterization of
disabled-1 expression in the developing chick retina. ACB Annual
Research Meeting, Banff, Alberta (2001).
Katyal, S., Bléoo, S.L., Packer, M.K. and Godbout, R.
Characterization of DDX1-interacting proteins. ACB Annual Research
Meeting, Banff, Alberta (2001).
Katyal, S., Bléoo, S.L., Packer, M.K. and Godbout, R. Interaction
screening of a DEAD box protein overexpressed in retinoblastoma and
neuroblastoma. 1st Canadian Developmental Biology Conference, Mont-Tremblant,
Quebec (2002).
Katyal, S., Packer, M.K. and Godbout, R. Disabled-1 is alternatively
spliced in the developing chick retina. 1st Canadian Developmental
Biology Conference, Mont-Tremblant, Quebec (2002).
Katyal, S., Packer, M.K. and Godbout, R. Interaction screening of a
DEAD box protein overexpressed in retinoblastoma and neuroblastoma.
ACB Annual Research Meeting, Banff, Alberta (2002).
Katyal, S. and Godbout, R. Identification of a biochemical function
for a DEAD box protein, DDX1,
amplified in retinoblastoma and neuroblastoma. RNA Trafficking,
Transport and Processing. Keystone Symposia, Snowbird, Utah (April,
2003).
Malhotra, G.S., Katyal, S. and Godbout, R. Identification of DDX1 RNA
substrates by RNA-immunoprecipitation (RNA-IP). The 36th Annual
Summer Students' Research Day, University of Alberta (October 25,
2003).
Katyal, S. and Godbout, R. Identification of a biochemical function
for a DEAD box protein, DDX1, amplified in retinoblastoma and
neuroblastoma. ACB Annual Research Meeting, Banff, Alberta (2003).
Katyal, S and Godbout, R. Alternative splicing modulates disabled-1
(Dab1) function in the developing chick retina. 2nd Canadian
Developmental Biology Symposium, Banff, Alberta (2004).
Cheung, J., Katyal, S. and Godbout, R. Understanding the role of the
DEAD box gene DDX1 in cancer. The 37th Annual Summer Students'
Research Day, University of Alberta (October 24, 2004) (poster
prize).
Kim, J. H., Sereda, C., Katyal, S. and Godbout, R. The role of AP-2
in retinal development and in retinoblastoma. ACB Annual Research
Meeting, Banff, Alberta (2004).
Gao, Z., Katyal, S., Monckton, E. and Godbout, R. Functional
characterization of Dab1 alternative splicing in chick retina. ACB
Annual Research Meeting, Banff, Alberta (2004).
Li, L., Katyal, S., Packer, M. and Godbout, R. Identification of DDX1
interacting proteins and DDX1 substrates. ACB Annual Research
Meeting, Banff, Alberta (2004).
Katyal, S. and Godbout, R. Alternative splicing modulates disabled-1
(Dab1) function in the developing retina. The American Society for
Cell Biology 44th Annual Meeting, Washington, D.C. (December 4-8,
2004).
Funding source:
Alberta Cancer Board studentship – Cyril Kay award (stipend and
research allowance)
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