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Research Project:
Brain fatty acid binding protein (B-FABP) is a member of a family
of proteins implicated in cancer formation and cellular
differentiation. B-FABP is believed to bind to fatty acid ligands
such as docosahexanoic acid (DHA) and deliver these ligands to
nuclear receptors implicated in transcriptional regulation.
Previous work from the lab has shown that B-FABP is expressed in
malignant glioma tumours and cell lines. As malignant glioma
tumours are highly aggressive and invasive, we need to find new
approaches for the treatment of these cancers. I am studying how
B-FABP expression affects the growth properties of malignant
glioma cells. I am particularly interested in determining whether
B-FABP expression leads to increased invasiveness and cell
motility.
Techniques: confocal microscopy, tissue
culture, motility assays, protein extraction, western blot
analysis, yeast two-hybrid analysis
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Presentations:
Mita, R., Roy, K., Rowe, J., Coles, J., Dabbagh, L., Hao, C and
Godbout, R. Subcellular localization of brain fatty acid-binding
protein in malignant glioma. Alberta Cancer Board Annual Research
Meeting, Banff, Alberta (2004).
Mita, R. and Godbout, R. Subcellular localization of brain fatty
acid-binding protein in malignant glioma. ACB Research Meeting,
Banff, Alberta (2005).
Mita, R., Coles, J.E., Sung, R. and Godbout, R. Subcellular
localization of brain fatty acid-binding protein in malignant glioma.
ACB Research Meeting, Banff, Alberta (2006)
Mita, R., Coles, J.E., Glubrecht, D. and Godbout, R. Expression of B-FABP
enhances migration of malignant glioma cells. The 6th Conference on
Lipid Binding Proteins, Vancouver, BC (2007)
Mita, R., Coles, J., Glubrecht, D. and Godbout, R. Role of brain fatty acid-binding protein in malignant glioma. Alberta Cancer Research Institute Research Meeting, Banff, Alberta (November 6-8, 2007). Oral presentation award
Mita, R., Coles, J., Glubrecht, D. and Godbout, R. Brain fatty acid-binding protein enhances migration in malignant glioma cells. 13th Biennial Canadian Neuro-Oncology Meeting, Banff, Alberta (May 16 – May 18, 2008).
Mita, R., Coles, J., Glubrecht, D. and Godbout, R. Brain fatty acid-binding protein enhances migration in malignant glioma cells. 17th Biennial Meeting of the International Society for Developmental Neuroscience, Asilomar, California (June 1-4, 2008). Published in Int. J. Dev. Neurosci. 26(8): 852 (P1.34) (2008).
Mita, R., Field, C. and Godbout, R. Brain fatty acid-binding protein enhances migration in malignant glioma cells. Alberta Cancer Research Institute Research Meeting, Banff, Alberta (November 25-27, 2008).
Mita, R., Field, C., Graham, K. and Godbout, R. Gene expression profiling of B-FABP-expressing malignant glioma cells. 2nd Annual Canadian Human Genetics Conference, Harrison Hot Springs, BC (May 24-27, 2009).
Mita, R., Field, C and Godbout, R. Role of brain fatty acid-binding protein and its fatty acid ligand DHA in malignant glioma. Lipid Signaling Pathways in Cancer. FASEB Summer Research Conferences, Carefree, Arizona (July 19-24, 2009).
Publications:
Mita, R. Coles, J.E., Glubrecht, D., Sung, R. Sun, X. and Godbout, R.
B-FABP-expressing radial glial cells – the malignant glioma
cell-of-origin? Neoplasia 9: 734-377 (2007).
Li. X., Glubrecht, D.D., Mita, R. and Godbout, R. Expression of AP-2d in the developing chick retina. Dev. Dyn. 237: 3210-3221 (2008).
Liu, R.-Z., Mita, R., Beaulieu, M., Gao, Z. and Godbout, R. Fatty acid binding proteins in brain development and diseases. Int. J. Dev. Biol. 54: 1229-1239 (2010).
Mita, R., Beaulieu, M.J., Field, C. and Godbout, R. Brain Fatty Acid-binding Protein and ?-3/?-6 Fatty Acids: Mechanistic Insight into Malignant Glioma Cell Migration. J. Biol. Chem. 285: 37005-37015 (2010).
Awards
CIHR Translational Research Training in Cancer fellowship
Poster award
- The 6th Conference on Lipid Binding Proteins, Vancouver, BC (2007)
Alberta Cancer Foundation Studentship
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