Stacey Knafelc, B. Sc., Research Technologist

I convocated from the University of Saskatchewan in 2007 with an Honors degree in the fields of Microbiology and Biochemistry. Following graduation, I was employed at the Saskatchewan Cancer Agency in Saskatoon, Saskatchewan. Upon moving to Edmonton I joined Dr. Mary Hitt’s lab in the Department of Oncology as a research technician.  

My most recent project involves Src and STAT3 gene silencing in ZR75 breast cancer cells using an engineered microRNA gene cluster. The efficiency of knockdown is monitored using Western Blotting, Northern Blotting and RT-PCR techniques and visualization by chemiluminescence. As well, work on a number of subcloning projects for the creation of new reporter and inducible plasmids has been undertaken. These are used in a variety of experimental procedures that include confocal microscopy, fluorescent microscopy, transient transfections, etc.